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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/08/2018 |
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Data da última atualização: |
12/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TOSCANO, J. H. B.; LOPES, L. G.; GIRALDELO, L. A.; SILVA, M. H. da; OKINO, C. H.; CHAGAS, A. C. de S. |
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Afiliação: |
João Henrique Barbosa Toscano, UNESP; Louyse Gabrielli Lopes, UNICEP; Luciana Aparecida Giraldelo, UNICEP; Matheus Henrique da Silva, UNICEP; CINTIA HIROMI OKINO, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.5, p.1253-1262, out. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Due to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results. MenosDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes,... Mostrar Tudo |
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Palavras-Chave: |
Genes de referência. |
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Thesagro: |
Haemonchus Contortus; Ovino. |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02463naa a2200229 a 4500
001 2094611
005 2018-09-12
008 2018 bl uuuu u00u1 u #d
100 1 $aTOSCANO, J. H. B.
245 $aIdentification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.$h[electronic resource]
260 $c2018
520 $aDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results.
650 $aSheep
650 $aHaemonchus Contortus
650 $aOvino
653 $aGenes de referência
700 1 $aLOPES, L. G.
700 1 $aGIRALDELO, L. A.
700 1 $aSILVA, M. H. da
700 1 $aOKINO, C. H.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv.45, n.5, p.1253-1262, out. 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 18 | |
| 1. |  | TOSCANO, J. H. B; GIRALDELO, L. A; SILVA, M. H; OKINO, C. H.; CHAGAS, A. C. de S. Associação entre perfil de citocinas inflamatórias em abomaso e resistência de ovinos morada nova ao haemonchus contortus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 20., 2018, Londrina, PR. Anais...Londrina, PR: CBPV, 2018. p. 313.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| 2. | | TOSCANO, J. H. B.; GIRALDELO, L. A.; SILVA. M. H.; OKINO, C. H.; CHAGAS, A. C. de S. Identificação de genes de referência para avaliação de expressão gênica em ovinos infectados com Haemonchus contortus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 20., 2018, Londrina, PR. Anais...Londrina, PR: CBPV, 2018. p. 373.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 3. | | CHAGAS, A. C. de S.; LOPES, L. G.; SILVA, M. H.; GIRALDELO, L. A.; TOSCANO, J. H. B.; OKINO, C. H.; BENAVIDES, M. V.; ESTEVES, S. N. Phenotypic characterization of the parasite resistance in Morada Nova sheep. In: ANNUAL MEETING OF THE EUROPEAN FEDERATION OF ANIMAL SCIENCE, 69., 2018, Dubrovnik, Croatia. Proceedings... Dubrovnik, Croatia: Wageningen Academic Publishers, 2018. p.308. 27-31 august 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 4. | | TOSCANO, J. H. B.; OKINO, C. H.; SANTOS, I. B. dos; GIRALDELO, L. A.; HAEHLING, M. B. VON; ESTEVES, S. N.; CHAGAS, A. C. de S. Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. Journal of Immunology Research, v. 2019, n. 3562672, p. 1-10, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | GIRALDELO, L. A.; LOPES, L. G.; SILVA, M. H. da; TOSCANO, J. H. B; BENAVIDES, M. V.; THOLON, P.; ESTEVES, S. N.; CHAGAS, A. C. de S. Impacto da infecção por nematoides gastrintestinais em cordeiros morada nova. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 20., 2018, Londrina. Anais...Londrina: CBPV, 2018. p. 374.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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| 6. | | TOSCANO, J. H. B.; LOPES, L. G.; GIRALDELO, L. A.; SILVA, M. H. da; OKINO, C. H.; CHAGAS, A. C. de S. Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus. Molecular Biology Reports, v.45, n.5, p.1253-1262, out. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 7. | | HAEHLING, M. B.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B.; ESTEVES, S. N.; BENAVIDES, M. V.; THOLON, P.; CHAGAS, A. C. de S. Is selection for resistance and resilience to Haemonchus contortus possible in Morada Nova sheep? Small Ruminant Research, v.201, aug. 2021, 106447. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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| 8. | | NOGUEIRA, J. A. P.; FIGUEIREDO, A.; DUARTE, J. L.; ALMEIDA, F. B. de; SANTOS, M. G.; NASCIMENTO, L. M.; FERNANDES, C. P.; MOURÃO, S. C.; TOSCANO, J. H. B.; ROCHA, L. M.; CHAGAS, A. C. de S. Repellency effect of Pilocarpus spicatus A. St.-Hil essential oil and nanoemulsion against Rhipicephalus microplus larvae. Experimental Parasitology, 215, 107919, aug. 2020. 5 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | OKINO, C. H.; CAMINOTTO, L. C. F.; KAPRITCHKOFF, R. T. I.; NICIURA, S. C. M.; ESTEVES, S. N.; SANTOS, I. B. DOS; TOSCANO, J. H. B.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. Comparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification. Veterinary Parasitology, v.300, 109592, dec. 2021. 5 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | OKINO, C. H.; NICIURA, S. C. M.; TOSCANO, J. H. B.; ESTEVES, S. N.; SANTOS, I. B. DOS; HAEHLING, M. B. VON; FIGUEIREDO, A.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. Ovine B-globin gene: a new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection. Veterinary Parasitology, v.294, june, 109434, 2021. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | TOSCANO, J. H. B.; SANTOS, B. dos; HAEHLING, M. B. VON; GIRALDELO, L. A.; LOPES, L. G.; SILVA, M. H. da; FIGUEIREDO, A.; ESTEVES, S. N.; CHAGAS, A. C. de S. Morada Nova sheep breed: resistant or resilient to Haemonchus contortus infection? Veterinary Parasitology, v. 276, supplement, 2019, 100019. 9 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 12. | | HAEHLING, M. B. V.; CRUVINEL, G. G.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B. dos; ESTEVES, S. N.; BENAVIDES, M. V.; BARIONI JUNIOR, W.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. Veterinary Parasitology, v. 279, 2020, 109053. 11 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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| 13. | | OKINO, C. H.; TOSCANO, J. H. B; GIRALDELO, L. A.; VON HAEHLING, M. B.; SANTOS, I. B. dos; ESTEVES, S. N.; FERRAZ JUNIOR, R. S.; CHAGAS, A. C. de S. Differential local immune responses against Haemonchus contortus in a resistant Brazilian sheep breed (Morada Nova). In: INTERNATIONAL VETERINARY IMMUNOLOGY SYMPOSIUM, 12., 2019, Seattle. proceedings... Seattle: AAVI, 2019. 114.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | TOSCANO, J. H. B.; OKINO, C. H.; VON HAEHLING; SANTOS, I. B. dos; GIRALDELO, L. A.; FIGUEIREDO, A.; ESTEVES, S. N.; FERRAZ JUNIOR, R. S.; BASSETO, C. C.; AMARANTE, F. T. do; CHAGAS, A. C. de S. Systemic immune responses associated to Haemonchus contortus resistance in a resistant Brazilian sheep breed (Morada Nova). In: INTERNATIONAL VETERINARY IMMUNOLOGY SYMPOSIUM, 12., 2019, Seattle. Proceedings... Seattle: AAVI, 2019. p.119.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | SANTOS, I. B. dos; MACIEL, W. G.; FELIPPELLI, G.; TOSCANO, J. H. B.; CRUZ, B. C.; CHAGAS, A. C. de S.; SOARES, V. E.; LOPES. W. D. Z.; COSTA, A. J. da; OLIVEIRA, G. P. de. Viability of Haemonchus placei parasitism in experimentally infected young goats. Veterinary Parasitology, v. 271, p. 64-67, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | OKINO, C. H.; TOSCANO, J. H. B.; LOPES, L. G.; SILVA, M. H.; GIRALDELO, L. A.; NICIURA, S. C. M.; BENAVIDES, M. V.; ESTEVES, S. N.; CHAGAS, A. C. de S. Immune cell profiles associated with resistance against Haemonchus contortus in Morada Nova sheep. In: ANNUAL MEETING OF THE EUROPEAN FEDERATION OF ANIMAL SCIENCE, 69., 2018, Dubrovnik, Croatia. Proceedings... Dubrovnik, Croatia: Wageningen Academic Publishers, 2018. p.310. 27-31 august 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 17. | | TOSCANO, J. H. B.; OKINO, C. H.; SANTOS, I. B. dos; GIRALDELO, L. A.; HAEHLING, M. B. von; ESTEVES, S. N.; BASSETTO, C. C.; AMARANTE, A. F. T. do; CHAGAS, A. C. de S. Local and systemic immune mediators of Morada Nova lambs with divergent Haemonchus contortus resistance phenotypes. Parasite Immunology, v. 42, e12790, 2020. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | FIGUEIREDO, A.; NASCIMENTO, L. M.; CALDAS, G. R.; ALBUQUERQUE, R. D. D. G.; SANTOS, M. G.; FALCÃO, D. Q.; TOSCANO, J. H. B.; ALEMÁN GAINZA, Y.; SILVA, M. H.; GIRALDELO, L. A.; LOPES, L. G.; ROCHA, L.; CHAGAS, A. C. de S. Atividade carrapaticida in vitro de nanoemulsão à base de óleo essencial de Ocotea elegans (Lauraceae) sobre larvas de Rhipicephalus (Boophilus) microplus. In: JORNADA PAULISTA DE PLANTAS MEDICINAIS "PROFESSOR DR. FRANCISCO JOSÉ DE ABREU MATOS", 13., 2017, Araraquara. Resumos... Araraquara: Unesp, 2017. p.55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 18 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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